molecular analyst software program Search Results


dynamics image quant software  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc dynamics image quant software
HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by <t>phosphorimaging</t> (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.
Dynamics Image Quant Software, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dynamics image quant software/product/Molecular Dynamics Inc
Average 90 stars, based on 1 article reviews
dynamics image quant software - by Bioz Stars, 2026-05
90/100 stars
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amber v18 software program  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc amber v18 software program
HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by <t>phosphorimaging</t> (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.
Amber V18 Software Program, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amber v18 software program/product/Molecular Dynamics Inc
Average 90 stars, based on 1 article reviews
amber v18 software program - by Bioz Stars, 2026-05
90/100 stars
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software program for densitometric analysis  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc software program for densitometric analysis
HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by <t>phosphorimaging</t> (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.
Software Program For Densitometric Analysis, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/software program for densitometric analysis/product/Molecular Dynamics Inc
Average 90 stars, based on 1 article reviews
software program for densitometric analysis - by Bioz Stars, 2026-05
90/100 stars
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molecular analyst software, fingerprinting plus  (Applied Maths)
90
Applied Maths molecular analyst software, fingerprinting plus
HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by <t>phosphorimaging</t> (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.
Molecular Analyst Software, Fingerprinting Plus, supplied by Applied Maths, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/molecular analyst software, fingerprinting plus/product/Applied Maths
Average 90 stars, based on 1 article reviews
molecular analyst software, fingerprinting plus - by Bioz Stars, 2026-05
90/100 stars
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imagequanta software program  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc imagequanta software program
HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by <t>phosphorimaging</t> (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.
Imagequanta Software Program, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imagequanta software program/product/Molecular Dynamics Inc
Average 90 stars, based on 1 article reviews
imagequanta software program - by Bioz Stars, 2026-05
90/100 stars
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molecular surface software program  (Accelrys)
90
Accelrys molecular surface software program
HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by <t>phosphorimaging</t> (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.
Molecular Surface Software Program, supplied by Accelrys, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/molecular surface software program/product/Accelrys
Average 90 stars, based on 1 article reviews
molecular surface software program - by Bioz Stars, 2026-05
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molecular analyst software version 2.1  (Kodak)
90
Kodak molecular analyst software version 2.1
HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by <t>phosphorimaging</t> (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.
Molecular Analyst Software Version 2.1, supplied by Kodak, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/molecular analyst software version 2.1/product/Kodak
Average 90 stars, based on 1 article reviews
molecular analyst software version 2.1 - by Bioz Stars, 2026-05
90/100 stars
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image space software program version 3.10  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc image space software program version 3.10
HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by <t>phosphorimaging</t> (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.
Image Space Software Program Version 3.10, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/image space software program version 3.10/product/Molecular Dynamics Inc
Average 90 stars, based on 1 article reviews
image space software program version 3.10 - by Bioz Stars, 2026-05
90/100 stars
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molecular simulation program  (ChemInnovation Software Inc)
90
ChemInnovation Software Inc molecular simulation program
HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by <t>phosphorimaging</t> (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.
Molecular Simulation Program, supplied by ChemInnovation Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/molecular simulation program/product/ChemInnovation Software Inc
Average 90 stars, based on 1 article reviews
molecular simulation program - by Bioz Stars, 2026-05
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software program  (Carestream Molecular Imaging)
90
Carestream Molecular Imaging software program
HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by <t>phosphorimaging</t> (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.
Software Program, supplied by Carestream Molecular Imaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/software program/product/Carestream Molecular Imaging
Average 90 stars, based on 1 article reviews
software program - by Bioz Stars, 2026-05
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cdocker molecular docking program in discovery studio 2.5 software package  (Accelrys)
90
Accelrys cdocker molecular docking program in discovery studio 2.5 software package
HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by <t>phosphorimaging</t> (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.
Cdocker Molecular Docking Program In Discovery Studio 2.5 Software Package, supplied by Accelrys, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdocker molecular docking program in discovery studio 2.5 software package/product/Accelrys
Average 90 stars, based on 1 article reviews
cdocker molecular docking program in discovery studio 2.5 software package - by Bioz Stars, 2026-05
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hybrid molecular docking program oedocking 3.4.0.2  (OpenEye Scientific Software Inc)
90
OpenEye Scientific Software Inc hybrid molecular docking program oedocking 3.4.0.2
HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by <t>phosphorimaging</t> (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.
Hybrid Molecular Docking Program Oedocking 3.4.0.2, supplied by OpenEye Scientific Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hybrid molecular docking program oedocking 3.4.0.2/product/OpenEye Scientific Software Inc
Average 90 stars, based on 1 article reviews
hybrid molecular docking program oedocking 3.4.0.2 - by Bioz Stars, 2026-05
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Image Search Results


HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by phosphorimaging (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.

Journal: RNA

Article Title: IGF2BP1 enhances HCV IRES-mediated translation initiation via the 3′UTR

doi: 10.1261/rna.1578409

Figure Lengend Snippet: HCV IRES-mediated translation initiation is enhanced by the HCV 3′UTR in vitro. (A,B, upper panel) Schematic representation of HCV 5′UTR and HCV 3′UTR bearing Luc reporter mRNAs. (Lower panel) Cytoplasmic Huh7 cell extract was used in cell-free translation reactions programmed with the [32P] trace-labeled mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luc activity is expressed as Luc units. Error bars denote the standard deviation from the mean of three different experiments. [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by phosphorimaging (Storm 860 Phosphor Imager; Molecular Dynamics, Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh7 cell extract in the presence of 2 mM cycloheximide for 1 and 15 min, as indicated. (D) [32P]-labeled HCV sORF mRNA (left panel) or HCVΔ3′UTR sORF mRNA (right panel) was incubated in Huh 7 cell extract in the presence of 2 mM cycloheximide and 5 mM GMP-PNP for 1 and 15 min, as indicated. (C,D) Translation initiation complexes were allowed to assemble for the time indicated and subsequently resolved by centrifugation on 5%–25% linear sucrose gradients. After fractionation from the bottom to the top of the gradient, the radioactivity was monitored, expressed as a percentage of incorporation, and plotted against the fraction number. RNA extracted from 22 fractions was analyzed on agarose gels and visualized by ethidium bromide staining. The positions of 18S and 28S rRNA are indicated. The distribution of rpS3 and eIF6 in the sucrose gradient fractions, determined by Western blotting, indicates the position of 80S ribosomes and ribosomal 60S and 40S subunits, respectively.

Article Snippet: The incorporation of [ 32 P] was determined by phosphorimaging (Storm 860 PhosphorImager; Molecular Dynamics Image Quant software) and expressed as a percentage of signal intensity.

Techniques: In Vitro, Labeling, Activity Assay, Standard Deviation, Extraction, Control, Agarose Gel Electrophoresis, Software, Incubation, Centrifugation, Fractionation, Radioactivity, Staining, Western Blot

IGF2BP1 enhances HCV IRES-mediated translation via the 3′UTR in rat primary hepatocyte extract. (A) Detection of IGF2BP1 and vinculin (control) in Western blot assays: (lane 1) recombinant IGF2BP1; (lane 2) cytoplasmic Huh 7 cell extract; (lane 3) cytoplasmic rat primary hepatocyte extract; (lane 4) total rat primary hepatocyte extract; and (lane 5) total extract of rat testis (antibody control). (B) Cytoplasmic extract of rat primary hepatocytes was used in cell-free translation reactions, programmed with [32P] trace-labeled reporter mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luciferase activity is expressed as relative Luc units. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by phosphorimaging (Storm 860 PhosphorImager; Molecular Dynamics Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (D) Reporter mRNAs HCV Luc or HCVΔ3′UTR Luc were incubated in cytoplasmic extracts of rat primary hepatocytes in the presence of increasing amounts of recombinant IGF2BP1. Luciferase activity is expressed as relative Luc units. Error bars denote the standard deviation from the mean of three different experiments. Translation was set to 100% in the absence of recombinant IGF2BP1. In the presence of recombinant protein, data were analyzed with the t-test (two-tailed), (***) p < 0.0005.

Journal: RNA

Article Title: IGF2BP1 enhances HCV IRES-mediated translation initiation via the 3′UTR

doi: 10.1261/rna.1578409

Figure Lengend Snippet: IGF2BP1 enhances HCV IRES-mediated translation via the 3′UTR in rat primary hepatocyte extract. (A) Detection of IGF2BP1 and vinculin (control) in Western blot assays: (lane 1) recombinant IGF2BP1; (lane 2) cytoplasmic Huh 7 cell extract; (lane 3) cytoplasmic rat primary hepatocyte extract; (lane 4) total rat primary hepatocyte extract; and (lane 5) total extract of rat testis (antibody control). (B) Cytoplasmic extract of rat primary hepatocytes was used in cell-free translation reactions, programmed with [32P] trace-labeled reporter mRNAs HCV Luc or HCVΔ3′UTR Luc for the times indicated. Luciferase activity is expressed as relative Luc units. Error bars denote the standard deviation from the mean of three different experiments. (C) [32P] trace-labeled mRNAs were extracted from the translation reaction at the time points indicated. [32P] trace-labeled 5′cap sORF mRNA was used as an extraction control. Extracted mRNAs were analyzed by agarose gel electrophoresis. The incorporation of [32P] was determined by phosphorimaging (Storm 860 PhosphorImager; Molecular Dynamics Image Quant software) and expressed as a percentage of signal intensity. Error bars denote the standard deviation from the mean of three different experiments. (D) Reporter mRNAs HCV Luc or HCVΔ3′UTR Luc were incubated in cytoplasmic extracts of rat primary hepatocytes in the presence of increasing amounts of recombinant IGF2BP1. Luciferase activity is expressed as relative Luc units. Error bars denote the standard deviation from the mean of three different experiments. Translation was set to 100% in the absence of recombinant IGF2BP1. In the presence of recombinant protein, data were analyzed with the t-test (two-tailed), (***) p < 0.0005.

Article Snippet: The incorporation of [ 32 P] was determined by phosphorimaging (Storm 860 PhosphorImager; Molecular Dynamics Image Quant software) and expressed as a percentage of signal intensity.

Techniques: Control, Western Blot, Recombinant, Labeling, Luciferase, Activity Assay, Standard Deviation, Extraction, Agarose Gel Electrophoresis, Software, Incubation, Two Tailed Test